Bioaugmentation Effect of Alcaligenes sp.2 and Isolates Bacillus sp.2 on Lowering Used Lubricating Oil- Contaminated Soil pH
Abstract
Abstract. This study aims to see the effect of biugmentation on pH values on contaminated oil used soil. In this study using Alcaligenes sp.2 isolates. and Bacillus sp.2 which was successfully isolated by Ahda and Fitri (2016). The bacterial formulation is a single isolate of Alcaligenes sp.2, Bacillus sp.2 and the Alcaligenes sp.2 and Bacillus sp.2 consortium. The variation in frequency of biaoaugmentation used in this study was 6 times biotechnology. Calculation of pH values is done once a week and carried out during the 6 weeks of the study period. The results of this study indicate that bioaugmentation influences the pH value of contaminated oil used soil. The type of bacteria that is the fastest in reducing and increasing pH in contaminated oil from used oil is a consortium of Alcaligenes sp.2 bacteria. and Bacillus sp.2.
Keywords: Bioaugmentation, Hydrocarbons, pH, Alcaligenes sp.2. and Bacillus sp.2.
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Adams F, Niyamugobudo A, Sylvetes OP. 2017. Bioremediation of crude oil contaminated soil using agriculturated wastes. Procedia Manufacturing. (8)459-464
Ahda Y & Fitri L. 2016. Karakterisasi Bakteri Potensial Pendegradasi Oli Bekas pada Tanah Bengkel di Kota Padang. Journal of Sainstek 8(2): 98-103
Basuki W. 2011. Bioremediasi Limbah Oli Bekas Oleh Lycinibacillus sphanericus TCP C 2.1. Jurnal Teknik Lingkungan. Vol. 12 No. 2.
Brown L.D, Cologgi D L, Gee K F, Ulrich A C. 2017. Bioremediation of oil spills on land. Oil Spill Science and Technology.
Burges A, Epelde L, Garbisu C. 2015. Impact of repeated single-metal and multi-metal pollution events on soil quality. Chemosphere 120 (2015) 8–15.
Charlena, Mas’ud ZA, Yani M, Sjahriza A, Tarigan JG. 2011. Biodegradasi limbah minyak berat menggunakan Isolat tunggal dan campuran dengan penambahan Alkilbenzena sulfonat linear. Seminar Nasional Kimia Terapan Indonesia 2011“Potensi Riset Kimia Terapan dalam Mendukung Pembangunan Iptek Berbasis Inovasi”
Cheng Y, Wang L, Faustorilla V, Megharaj M, Naidu R, Chen Z. 2017. Integrated electrochemical treatment systems for facilitating the bioremediation of oil spill contaminated soil. Chemosphere 175 (2017) 294e299
Garbisu C, Olatz G, Lur E, Elisabeth G, Itziar A. (2017). Plasmid-Mediated Bioaugmentation for the Bioremediation of Contaminated Soils. fronties of microbiology.8:1966.
Goudarzi GG, Sahar , Alavi N. 2018. Association between cancer risk and polycyclic aromatic hydrocarbons’ exposure in the ambient air of Ahvaz, southwest of Iran. International Journal of Biometeorology.
Gosalam, S., Akbar T., dan Silvana J.L. (2008). Uji Kemampuan Bakteri dari Perairan dalam Mendegradasi Senyawa Minyak Solar. Jurnal Ilmu Kelautan dan Perikanan Universitas Hasanuddin.
Hafiluddin. 2011. Bioremediasi Tanah Tercemar minyak Dengan Teknik Bioaugmentasi dan Biostimulasi. Emryo Vol. 8 No. 1
Ismayana, A., dkk. 2012. Faktor Rasio C/N Awal dan Laju Aerasi pada Proses Co-Composting Bagasse dan Blotong. Jurnal Teknologi Industri Pertanian. Departemen Industri Pertanian Fakultas Pertanian IPB. Bogor.
Kalantary RR, Baadkoubi A, Mohseni-Bandpi A, Esrafili A, Baneshi MM, Jorfi S. 2013. Modification of phenanthrenes biodegradation with humic compounds. Soil Sed Cont; 22:185-198.
Karyab H, Yunesian M, Nasseri S, Mahvi AH, Ahmadkhaniha R, Rastkari N, 2013. Policiclic aromatic hidrocarbonin drinking water of tehran, Iran. J Environ Health Sci Eng 11 (25)
Liu Y, Chen L, Zhao J, Wei Y, Pan Z, Meng XZ, Huang, Q, and Li W. 2010. Polycyclic aromatic hydrocarbons in the surface soil of Shanghai, China: Concentrations, distribution and sources. Organic Geochemistry. 41, 355-362.
Mohanti S, Jasmine J, & Mukherji S. 2013. Practical Considerations and Challenges Involved in urfactant Enhanced Bioremediation of Oil. BioMed Research International
Nugroho A. 2006. Biodegradasi ‘Sludge’ Minyak Bumi Dalam Skala Mikrokosmos.Makara Teknologi. 10 (2): 82-89
Nuryana D. 2017. Review: Bioremediasi Pencemaran Minyak Bumi. JEEE Vol. 6 No. 2
Prayitno, J. (2017). The Effectiveness of Microbial Consortia for Bioremediation of Oil- Contaminated Soil Using Bench-Scale Landfarming Technology. Jurnal Teknologi Lingkungan. Vol. 18, No 2, Juli 2017, 208-215..
Rojo, F., (2010), Carbon catabolite repression in Pseudomonas: optimizing metabolic versatility and interactions with the environ-ment. FEMS Microbiol. Rev. 34: 658-684
Shen W, Zhu N, Cui J, Wang H, Dang Z, Wu P, Luo Y, Shi C. 2016. Ecotoxicity monitoring and bioindicator screening of oil-contaminated soil during bioremediation.Ecotoxicology and Environmental Safety. 124(2016)120–128.
Sulistyorini dan Munawar Ali., MT. 2015. Bioremediasi Dengan Pseudomonas Putida Terhadap Pencemaran Tanah Minyak Bumi Dengan Bioaugmentasi. Jurnal Envirotek Vol. 10 No. 1
Yao Y, Lu Z, Zhu F, Min H, Bian C. 2013. Successful bioaugmentation of an activated sludge reactor withRhodococcus sp. YYL for efficient tetrahydrofuran degradation. Journal of Hazardous Materials (261) 550– 558
Yolantika H, Periadnadi, Nurmiati. 2015. Isolasi Bakteri Pendegradasi Hidrokarbon di Tanah Tercemar Lokasi Perbengkelan Otomotif. Jurnal Niologi Universitas Andalas. 4(3): 153-157.
DOI: http://dx.doi.org/10.24036/5793RF00