KUALITAS DNA HASIL ISOLASI DARI BEBERAPA BAGIAN BATANG RAMBUT UNTUK BAHAN ANALISIS DNA FORENSIK

Abstract

Hair and blood spots are two examples of forensic evidence that may be found at the crime scene. For DNA fingerprint analysis, those samples can be used as sources of DNA. Part of hair that most often used in DNA fingerprint analysis is root of hair because they contain much DNA. But in fact, samples found in the field are not always intact, such as hair without a root. Therefore, DNA isolation should be done from the hair shaft. The same thing happens to blood cells, in which fresh blood is a best source of DNA for isolation. However, the reality on the scene, which is often found dried blood spots that should be sought as much as possible to get DNA from the samples available. The purpose of this study is to find the procedure of DNA isolation of the hair shaft and dried blood spots on the fabric and to determine the quality of DNA isolation. The study was a descriptive study with the working stages are the isolation of DNA from the hair shaft and dried blood spots on the fabric by modifying the procedure of DNA isolation from sources of fresh cells, DNA amplification using the primers Gaeno and STR D7S820, and electrophoresis of PCR product to analyse the quality of DNA amplification. The results showed that DNA could only be isolated from the hair shaft close to the roots. This is evidenced by the formation of PCR products amplified using primers Gaeno and STR D7S820. DNA isolation that is performed in the middle and the end of the hair shaft does not get the DNA, as evidenced by the unsuccessful amplification using template DNA derived from both parts of the hair. DNA isolation is also successfully carried out from dried blood spots with a volume of 300 ml, 400 ml and 500 ml. DNA amplification using the primers Gaeno gave positive results using template DNA from dried blood of the three kind volume, but the amplification of STR using D7S820 primers produce only positive on the source of DNA isolated from 500 ml of dried blood spots.

Keyword: Hair, blood spots, DNA isolation