Optimasi Komposisi Fasa Gerak dan pH Buffer Asetat Pada Analisa Zat Warna Sintetik Rhodamin B dan Ponceau 4R Menggunakan Metoda HPLC

Abstract

One of the food addictives that is often used by many people is dye. More synthetic dyes is used in food and beverage than natural dyes time by time. According to Regulation of Health Ministry No.239/Menkes/Per/VI/85, Rhodhamin B is synthetic dye which is banned because it is a carcinogen.  However, Ponceau 4R is allowable synthetic dye. The way of using Ponceau 4R must be based on regulation of the Indonesia Health Ministry No. 722/Menkes/per/IX/88; 70 mg/kg for beverage and 300 mg/kg for food. The purpose of this research is to find the optimum condition of the mobile phase composition and pH buffer acetate.   The analysis of the Rhodamin B and Ponceau 4R were done by optimalizing the mobile phase composition and pH acetate buffer with methanol mobile phase: acetate buffer and stationary phase ODS C18 column used the HPLC method of the UV-Vis detector. The result of the optimization pH acetate buffer was pH 6 acetate buffer with a ratio composition of methanol: buffer (80:20) with a wavelength of 540 nm. Retention times obtained for Rhodamine B was 5,048 minutes and Ponceau 4R was 2,528 minutes. There were four samples containing dye Ponceau 4R and none containing dye Rhodamine B were found by using the HPLC method from the ten samples tested