KEBERADAAN CENDAWAN DARK SEPTATE ENDOPHYTE (DSE) PADA SISTEM PERAKARAN BENIH SHOREA SELANICA

Abstract

ABSTRACT

Fungi have a variety of symbiotic interactions with plant roots, ranging from antagonism to mutualism. The mycorrhizal symbiosis between plants and fungi is universal, and most plant species are dependent on this symbiosis, including many economically important forest trees and crops. The another type of symbiosis is non- mycorrhizal association including fungal root endosymbiont. Dark Septate Endophyte (DSE) is one of non-mycorrhizal association between fungal and root plant. The occurance of DSE in root’s system of family of Dipterocarpaceae, especially Shorea selanica was not known yet. So, the research was conduct to analize the occurance of DSE in roots system of S. selanica seedling. DSE was found in 84% of the seedling observed and colonized 7.5 - 83.3% of its root segments. Under light microscopic observation, DSE occurs in roots system as chain-like hyphae inter- and intracellularly, microsclerotia intracellularly and short brown branched hyphae on root surface. These structures may either have brown or blue colour after tryphane blue staining.

Keywords: Root Endosymbiont, Dark Septate Endophyte, Shorea Selanica.