PERKIRAAN MASSA MOLEKUL ENZIM PENDEGRADASI INULIN DARI BAKTERI TERMOFILIK Bacillus licheniformis DAN AKTIVITAS ENZIM PADA GRANULA INULIN

Abstract

ABSTRACT

Inulin degrading enzymes from thermophilic bacteria is potential developed in food industry to produce fructose and fructo-oligosaccharides (FOS) prebiotics. In this study, inulin degrading enzymes from thermophilic bacterium Bacillus licheniformis was purified partially by ammonium sulfate. Molecular mass of inulin degrading enzymes was determined on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE). Molecular mass of inulin degrading enzymes from Bacillus licheniformis was predicted 57.2 kDa. The enzymes activity was conducted on inulin granula from chicory. The enzyme was active on inulin granula. Β-sandwich domain of the enzymes was thought to degrade inulin granula which is insoluble polysaccharides.

Keywords; Bacillus Licheniformis, Enzim Pendegradasi Inulin, Inulinase, SDS-PAGE, GH32