Placental Histopathology Following Rubraxanthone Administration in Mice (Mus musculus): A Toxicological Study

Elsa Badriyya, Rola Karnila Sari, Dian Ayu Juwita, Almahdy A

Abstract


Rubraxanthone is a primary compound isolated from the bark of the tamarind plant (Garcinia cowa Roxb), which has biological activity as an anti-cancer, anti-cholesterol, anti-inflammatory, anti-bacterial, and anti-thrombocyte effect. However, its safety profile concerning reproductive health, particularly placental development, remains poorly understood. This study aims to examine the impact of varying doses of Rubraxanthone on placental histopathological changes. Rubraxanthone was given in suspension form to 20 mice divided into four groups during the organogenesis period. Three treatment groups received 2, 4, and 8 mg/kgBW doses. Laparotomy was conducted on the 18th day of pregnancy. Histopathological changes in the placenta were observed descriptively. The study demonstrated that the dosage of Rubraxanthone administered influenced the histopathological changes in the placenta. The treatment group received rubraxanthone at a 4 mg/kgBW dose, which resulted in observed stasis and thrombosis. Meanwhile, the group given rubraxanthone at a dose of 8 mg/kgBW exhibited more severe damage, including necrosis, edema, and the formation of cavities. Based on the research, it was concluded that increasing the Rubraxanthone given during the study would affect the level of damage from histopathological changes in the placenta.


References


Rahmatullah SW, Susiani EF, Pahlevi MR, Kurniawan G, Maida K. Antipyretic Activity Test of the N-Hexane Fraction of Lime Fruit Peel (Citrus Aurantifolia (Christm.) Swing) using Dpt-Hb-Hib Vaccine Induction in Male Mice Strains. J Insa Farm Indonesia.2021;4(1):147–57.

Wahyuni FSEF, Husni E. Study of the Cytotoxic Effect of Fractionated Ethanol Extract of Kandis Asam Fruit Peel (Garcinia cowa Roxb.) on T47d Breast Cancer Cells using the Microtetrazolium (MTT) Method. Latest Pros of Semin Nas Perkemb Science Farm and Clin III. 2013;78–87.

Ilham FY, Wahyuni FS, Husni E. Cytotoxic Effect Test Results of Fractionation of Ethanol Extract of Kandis Acid Root (Garcinia cowa Roxb.) on T47d Breast Cancer Cells using the MTT Method. Latest Pros of Semin Nas Perkemb Science Farm and Clin III. 2013;88–92.

Kurniawan RN, Ersam T. Isolation of Rubrasanton and Parvifolisantone C Compounds from Garcinia Tetrandra Pierre Stem Bark Extract. J ITS Science and Arts. 2018;7(1):10–4.

Wahyuni, A H. Subchronic Toxicity Test of the Ethyl Acetate Fraction of Kandis Asam Fruit Peel (Garcinia cowa Roxb.) on Liver and Kidney Function of Female White Mice. J Science and Farm Clin. 2017;3(2):202–12.

Ritthiwigrom T. Chemical Constituents and Biological Activities of Garcinia cowa Roxb. Maejo Int J Sci Technol. 2013;7(2):212–31.

Dachriyanus, Sartika L, Kesuma M, Mukhtar M. Effect of Rubraxanthon Compound on Total Cholesterol, Triglyceride, HDL and LDL Levels in the Blood of Male White Mice. J Sci Tech Far. 2006;11(1):12–5.

Wahyuni F, Ali D, Lajis N, Dachriyanus. Anti-inflammatory Activity of Iso lated Compounds from the Stem Bark of Garcinia cowa Roxb. Pharmacogn J. 2017;9(1):55–7.

Jantan I, Pisar M, Idris M, Taher M, Ali R. In Vitro Inhibitory Effect of Rubraxanthone Isolated from Garcinia parvifolia on Platelet Activating Factor Receptor Binding. Planta Med. 2002;

Alen Y, Safitri N, Ali AM, Ladjis NH, Sargent M V. Rubraxhantone from Garcinia forbesii King. and its bioactivity. 2008;1(2):192–201.

He M, Skaria P, Kreutz K, Chen L, Hagemann IS, Carter EB, et al. Histopathology of Third Trimester Placenta from SARS-CoV-2-Positive Women. Fetal Pediatr Pathol [Internet]. 2022;41(3):403–12. Available from: https://doi.org/10.1080/15513815.2020.1828517

Abebe MS, Asres K, Bekuretsion Y, Woldekidan S, Sisay B, Seyoum G. Prenatal Developmental Toxicity and Histopathological Changes of the Placenta Induced by Syzygium guineense Leaf Extract in Rats. J Toxicol. 2022;2022.

Haryanto, Pertiwi W, Ihsani N. Estrus Cycle of Virgin Female Mice (Mus musculus) BALB/c Strain after Exposure to Various Types of Sound. J Sci Technol Entrepreneursh. 2019;1(2).

Organization W. Obesity. World Heal Organ. 2000;

BPOM. Food and Drug Supervisory Agency Regulation Number 10 of 2022 concerning Guidelines for In Vivo Preclinical Toxicity Testing. Republic of Indonesia Food and Drug Supervisory Agency. 2022;1–220.

A A. Experimental Teratology. Padang: Andalas University Press; 2012. 1–60 p.

Maghfyra H. Teratogenic Effects of Antituberculosis Drugs (OAT) Combination HRZE. 2022.

Simbolon SEB, Durry M, Lintong P. Histopathological Features of the Placenta in Pregnancy with Preeclampsia. J e-Biomedik. 2013;1(2):1069–74.

Lopez A, Martinson SA. Pathologic Basis of Veterinary Disease. Sixth Edit. 2017. 471–560 p.




DOI: https://doi.org/10.24036/bsc.v9i1.131616

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