The Ability of Phosphate Solubilizing Bacteria in Enhacing The Growth of Tomato Plants (Lycorsicon esculentum Mill.)

Linda Advinda, Violita Violita, Mentari Larashinda, Desi Susanti, Lati Jofanita

Abstract


Tomato (Lycopersicon esculentum Mill.) is a type of vegetable that is much needed the Indonesians. The high demand for tomatoes makes them very salable in the market, so the growth of tomatoes must be increased. Pseudomonas fluorescens is included in PGPR, one of the bacteria that lives as a saprophyte in plant roots and functions to increase plant growth. P. fluorescens is a phosphate solubilizing bacteria that has the ability to solubilizing phosphate bound to other elements so that it can be available to plants. The purpose of this study was to determine the ability of P. fluorescens in solubilizing phosphate, and to test its ability to increase tomato growth. This research is descriptive and experimental research. The design used was a Randomized Block Design (RBD) with 6 treatments (P. fluorescens isolates PfPb1, PfPb3, PfPj1, PfMs, PfCas and LAHLS1) and 3 replicates. The data were analyzed using the Analysis of Variance (ANOVA) test and continued with the DNMRT further test at the 5% level. The results showed that all P. fluorescens isolates were able to solubilizing phosphate with the formation of a clear zone, but could not increase the growth of tomato plants. 


Keywords


Fluorescent pseudomonads, PGPR, phosphate, tomato (Lycopersicon esculentum Mill.)

References


Advinda, L., T. Habazar, A. Syarif, Mansyurdin, dan P.D. Putra. 2007. Aktivitas Enzim Pertahanan Bibit Pisang yang Diinduksi dengan Pseudomonas fluorescens. Jurnal Ilmiah Konservasi Hayati, 3(2): 87–94.

Advinda, L. 2018. Dasar-Dasar Fisiologi Tumbuhan. Yogyakarta: Deepublish.

Advinda, L. 2020. Pseudomonas Fluorescens Agens Biokontrol Blood Disease Bacteria (BDB) Tanaman Pisang. Yogyakarta: Deepublish.

Badan Pusat Statistik Sumatera Barat. 2020. Konsumsi Sayur-Sayuran Per Kapita Per Minggu di Sumatera Barat 2018-2020 Menurut Survei Sosial Ekonomi Nasional (Publikasi Pola Konsumsi Makanan Penduduk Sumatera Barat).

Badan Pusat Statistik. 2021. Produksi Tanaman Sayuran 2021. Badan Pusat Statistik Indonesia.

Bernadus, T. dan W. Wahyu. 2002. Bertanam Tomat. Jakarta: Agromedia Pustaka.

Coyle, M.B. 2005. Manual of Antimicrobial Susceptibility Testing. American Society for Microbiology.

Daniel, A.I., A.O. Fadaka, A. Gokul, O.O. Bakare, O. Aina, S. Fisher, A.F., Burt, V. Mavumengwana, M. Keyster, and A. Klein. 2022. Biofertilizer: The Future of Food Security and Food Safety. Microorganisms, 10(6): 1–16.

Elfiati, D. 2005. Peranan Mikroba Pelarut Fosfat Terhadap Pertumbuhan Tanaman. Respotory, 2(1): 1–10.

FAO. 1993. The State of Food and Agriculture. Soil Science, 95(1): 284.

Fitriatin, B.N., A. Yuniarti, O. Mulyani, F.S. Fauziah, and M.D. Tiara. 2009. Pengaruh Mikroba Pelarut Fosfat dan Pupuk P terhadap P Tersedia, Aktivitas Fosfatase, P Tanaman dan Hasil Padi Gogo (Oryza sativa L.) pada Ultisol. Agrikultura, 20(3): 210–215.

George, T., P. Gregory, M. Wood, D. Read, and R. Buresh. 2002. Phosphatase Activity and Organic Acid In The Rhizosphere Of Potential Agroforestry Speciesand Maize. Soil Biology and Biochemistry, 34(10): 1487–1494.

Handayanto, E., dan K. Hairiah. 2007. Biologi Tanah Landasan Pengelolaan Tanah Sehat. Yogyakarta: Pustaka Adipura.

Haq, S.U., G. Ayub, S. Saleem, A. Khan, N. Khan, and S. Zubair. 2020. Effect of phosphorus with and without phosphate solubilizing bacteria on growth and yield of tomato. Pure and Applied Biology, 9(3): 2111–2121.

Ikhajiagbe, B., G.O. Anoliefo, O.F. Olise, F. Rackelmann, M. Sommer, and I.J. Adekunle. 2020. Major phosphorus in soils is unavailable, yet critical for plant development. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 12(3): 500–535.

Istiqomah, I., L.Q. Aini, and A.L. Abadi. 2017. Kemampuan Bacillus subtilis dan Pseudomonas fluorescens dalam Melarutkan Fosfat dan Memproduksi Hormon IAA (Indole Acetic Acid) Untuk Meningkatkan Pertumbuhan Tanaman Tomat. Buana Sains, 17(1): 75-84.

Landa, B.B., HAE De Werd, B.B. McSpadden Gardener, and D.M. Weller. 2002. Comparison of three methods for monitoring populations of different genotypes of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens in the rhizosphere. Phytopathology, 92(2): 129–137.

Purwaningsih, S. 2012. Isolasi, Populasi dan Karakterisasi Bakteri Pelarut Fosfat Pada Daerah Perakaran dan Tanah Dari Bengkulu, Sumatera. Jurnal Teknologi Lingkungan, 13(1): 101–108.

Rohmah, F., Y.S. Rahayu, and Yuliani. 2013. Pemanfaatan Bakteri Pseudomonas fluorescens, Jamur Trichoderma harzianum dan Seresah Daun Jati (Tectona grandis) untuk Pertumbuhan Tanaman Kedelai pada Media Tanam Tanah Kapur. LenteraBio, 2(2): 149–153.

Sharma, A., R. Jansen, M. Nimtz, B.N. Johri, and V. Wray. 2007. Rhamnolipids from the rhizosphere bacterium Pseudomonas sp. GRP3 that reduces damping-off disease in chile and tomato nurseries. Journal Natural Products, 70(6): 941–947.

Susanti, D., dan L. Advinda, 2021. Seleksi Beberapa Isolat Pseudomonas fluorescens Dalam Kemampuannya Melarutkan Fosfat. Prosiding SEMNAS BIO Biologi, FMIPA Universitas Negeri Padang. ISSN: 2809-8447, Padang Indonesia.

Stephen J., C.R. Ivonne D., H. Ronald J., S Robert L., M. Yvette S., S. Susan E., O. José H., and S. Carol. 2005. Manual of Antimicrobial Susceptibility Testing. Page News.Ge. Seattle Washington: University of Washington.

Vessey, J. 2003. Plant Growth Promoting Rhizobachteria as Biofertilizer. Plant and Soil, 225: 571–586.

Wijayanti, E. dan A.D. Susila. 2013. Pertumbuhan dan Produksi Dua Varietas Tomat (Lycopersicon esculentum Mill.). Bul. Agrohorti, 1(1): 104–112.

Yadav, A., K. Yadav, and A. Vashistha. 2016. Phosphate solubilizing activity of Pseudomonas fluorescens PSM1 isolated from wheat rhizosphere. Journal of Applied and Natural Science, 8(1): 93–96.

Yanti, Y., Gustian, and R. Haliatur. 2009. Aplikasi Agen Hayati Pseudomonas fluorescens sebagai Penginduksi Ketahanan Untuk Meningkatkan Produksi Tanaman Cabai Terhadap Penyakit Virus Kuning di Kecamatan Kuranji Kotamadya Padang. Warta Pengabdian Andalas, 15(1):47–57.




DOI: https://doi.org/10.24036/bsc.v9i1.131495

Refbacks

  • There are currently no refbacks.


Copyright (c) 2025 Linda Advinda, Violita Violita, Mentari Larashinda, Desi Susanti, Lati Jofanita

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Bioscience is Indexed By: