Antioxidant Activity, Total Phenolic and Flavonoid Content of Honey Bee
Abstract
Honey is a natural product that has many benefits,for living organism. The composition of honey is very diverse and depends on the type of honey and the region of origin. Bioactive compounds in honey including phenolics and flavonoids cause honey to having antioxidant activity. This study aims to identify honey bees, determine the chemical properties of honey based on the parameters of water content, acidity, and reducing sugar, and analyze the antioxidant activity and phenolic and flavonoid content of honey from Bukit Tiga Puluh National Park Riau and Prabumulih South Sumatra. The research using descriptive method were morphological identification of bees, testing parameters of honey bee chemical properties, DPPH antioxidant test to obtain IC50 (Inhibition consenteration) values, total phenolic and flavonoid levels. The identified bees are Apis mellifera (from Riau) and A. dorsata (from Prabumulih). This essay showed that the determination of water content, acidity, reducing sugar, both honeys fulfill SNI 8664:2018 except the reducing sugar of A. dorsata honey bee. A. dorsata honey bee has antioxidant activity higher IC50 value (590 mg/L) than A. mellifera honey (678 mg/L). Total phenolic (TPC) and flavonoid (TFC) of A. mellifera honey were 7.51 mg GAE/g and 0.06 mg QE/g, respectively higher than A. dorsata honey bee (3.35 mg GAE/g and 0.42 mg QE/g).
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Adalina, Y. (2017). Kualitas madu putih asal Provinsi Nusa Tenggara Barat. Posiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 3(2), 189–193. https://doi.org/10.13057/psnmbi/m030204
Al-Mamary, M., Al-Meeri, A., & Al-Habori, M. (2002). An tioxidant activities and total phenolics of different types of honey. Nutrition Research, 22(9), 1041–1047. https://doi.org/10.1016/S0271-5317(02)00406-2
Ansyarif, A. R. (2018). Kajian sifat fisikokimia madu hutan (Apis dorsata) dari daerah Maros, Pangkep dan Gowa Sulawesi Selatan. Skripsi, Universitas Islam Negeri Alauddin Makassar.
Arnanda, Q. P., Nuwarda, R. F., Studi, P., Farmasi, S., Farmasi, F., & Padjadjaran, U. (2019). Penggunaan radiofarmaka teknesium-99M dari Senyawa glutation dan senyawa flavonoid sebagai deteksi dini radikal bebas pemicu kanker. Farmaka, 17(2), 236–243.
Asih, D. J., Warditiani, N. K., & Wiarsana, I. G. S. (2022). Review artikel: Aktivitas antioksidan ekstrak Amla (Phyllanthus emblica/Emblica officinalis). Jurnal Ilmiah Multidisplin Indonesia, 1(6), 674–687.
Badan Standarisasi Nasional. (2018). Standar nasional Indonesia (SNI) 8664:2018 madu. www.bsn.go.id
BadanPusatStatistik. (2020). Statistik produksi kehutanan Indonesia 2020. Badan Pusat Statistik. Jakarta. www.bps.go.id
Cahyaningrumm, P. L., Ayurweda, P. K., & Indonesia, U. H. (2019). Aktivitas antioksidan madu ternakan dan madu kelengkeng sebagai pengobatan alami. E-Journal Widya Kesehatan, 1(1), 1–6.
Chayati, I., & Miladiyah, I. (2014). Kandungan komponen fenolat, kadar fenolat total, dan aktivitas antioksidan madu dari beberapa daerah di Jawa dan Sumatera. Media Gizi Mikro Indonesia, 6(1), 11–24.
Chua, L. S., Rahaman, N. L. A., Adnan, N. A., & Eddie Tan, T. T. (2013). Antioxidant activity of three honey samples in relation with their biochemical components. Journal of Analytical Methods in Chemistry, 1–8. https://doi.org/10.1155/2013/313798
Da Silva, P. M., Gauche, C., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2016). Honey: chemical composition, stability and authenticity. Food Chemistry, 309–323. https://doi.org/10.1016/j.foodchem.2015.09.051
Engel, M. S. (2012). The honey bees of Indonesia (Hymenoptera: Apidae). Treubia- a Journal on Zoology of the Indo - Australian Archipelago, 39, 41–49.
Evahelda. (2017). Sifat fisik dan kimia madu dari nektar pohon karet di Kabupaten Bangka Tengah. Agritech, 37(4), 363–368.
Fahay, A. J., Rijal, S., Arsal, A. S. F., Kanang, I. L. D., & Dwimartyono, F. (2022). Fakumi medical journal. Fakumi Medical Journal, 2(10), 687–693. https://fmj.fk.umi.ac.id/index.php/fmj
Fatma, I. I., Handayani, S., Widodo, S., & Suedy, A. (2017). Uji kualitas madu pada beberapa wilayah budidaya lebah madu d Kabupaten Pati. Jurnal Akademika Biologi, 6(2), 58–65. https://ejournal3.undip.ac.id/index.php/biologi/article/view/19538
Gairola, A., Tiwari, P., & Tiwari, J. K. (2013). Physico-chemical properties of Apis cerana-indica F. honey from Uttarkashi district of Uttarakhand, India. Journal of Global Biosciences, 2(1), 20–25. http://mutagens.co.in/jgb.html
Gheldof, N., XH, W., & NJ, E. (2003). Buckwheat honey increases serum antioxidant capacity in humans. Agric Food Chem, 51(5), 1500–1505. https://doi.org/10.1021/jf025897t
Goslinski, M., Nowak, D., & Szwengiel, A. (2021). Multidimensional comparative analysis of bioactive phenolic compounds of honeys of various origin. Multidisciplinary Digital Publishing Institute, 10(530), 1–14. https://doi.org/https://doi.org/10.3390/antiox10040530
Gunawan, R., Erwin, & Syafrizal. (2018). Uji fitokimia dan penentuan aktivitas antioksidan dari madu Trigona incisa. Jurnal Atomik, 3(1), 18–21.
Hadisoesilo, S. (2001). Keanekaragaman spesies lebah madu asli Indonesia. Biodiversitas, 2(1), 123–128. https://doi.org/10.13057/biodiv/d020107
Handayani, T. H. (2022). Aktivitas antioksidan, total fenolik, dan total flavonoid madu Apis mellifera dari hutan akasia (Accacia crassicarpa) Riau, Indonesia dengan beberapa perlakuan pengeringan. Jurnal Biologi Indonesia, 18(2), 231–243. https://doi.org/10.47349/jbi/18022022/231
Husna, I. S. H., Santoso, H., & Lisminingsih, R. D. (2020). Perbandingan kadar gula nektar dan kadar madu yang dihasilkan oleh Lebah ( Apis mellifera ) di Pusat Perlebahan Kota Batu. Jurnal Ilmiah Sains Alami, 2(2), 39–44.
Jasmi. (2013). Tumbuhan yang dikunjungi lebah pekerja Apis (Hymenoptera: Apidae) di Sumatera Barat. Jurnal Sainstek, 5(1), 38–45. https://doi.org/ISSN: 2085-8019
Juniarti, P. I. (2019). Pengenalan koloni lebah madu. (Artikel) Dinas Lingkungan Hidup dan Kehutanan Provinsi Daerah Istimewa Yogyakarta. https://dlhk.jogjaprov.go.id/pengenalan-koloni-lebah-madu
Kaligis, M. I. G., & Mokosuli, Y. S. (2022). Characteristics and flavonoid content of honey Apis dorsata Binghami from the Manembo forest of South Minahasa. Jurnal Biologi Tropis, 22(4), 1420–1430. https://doi.org/10.29303/jbt.v22i4.4247
Khabibi, J., Albayudi, & Ginting, D. J. (2022). Kualitas madu dari 3 spesies lebah penghasil madu. Jurnal Sliva Tropika, 6(1), 43–50.
Kwatrina, R. T., & Mukhtar, A. S. (2006). Kriteria dan indikator penetapan zonasi Taman Nasional Bukittigapuluh. Journal Penelitian Hutan dan Konservasi Alam, 3(5), 585–606.
Mahani, Savitri, S. R., & Subroto, E. (2022). Hubungan kadar flavonoid dan aktivitas antioksidan madu dari berbagai provinsi di Indonesia. Jurnal Sains Dan Teknologi Pangan, 7(4), 5255–5268.
Micheler, C. D. (2007). The bees of the world second edition. The John Hopkins University Press.
Minarti, S., Jaya, F., & Merlina, P. (2016). Pengaruh masa panen madu lebah pada area tanaman kaliandra (Calliandra calothyrsus) terhadap jumlah produksi kadar air, viskositas dan kadar gula madu. Jurnal Ilmu Dan Teknologi Hasil Ternak, 11(1), 46–51. https://doi.org/10.21776/ub.jitek.2016.011.01.1.5
Moniruzzaman, M., Sulaiman, S. A., Khalil, I., & Gan, S. H. (2013). Evaluation of physicochemical and antioxidant properties of sourwood and other Malaysian honeys : a comparison with manuka honey. Chemistry Central Journal, 7(138), 1–12.
Nidup, T., & Dorji, P. (2016). The honey bees (Hymnoptera: Apidae) of Bhutan with a key to the Apis species. Bio Bulletin, 2(2), 1–7. www.biobulletin.com
Nur’aini, & Purwanto, H. (2021). Morphology, morphometrics, and molecular characteristics of Apis cerana and Apis nigrocincta from Central Sulawesi, Indonesia. Jurnal Biologi Tropis, 21(2), 368–382. https://doi.org/http://dx.doi.org/10.29303/jbt.v21i2.2614
PemerintahKotaPrabumulih. (2023). Selayang pandang geografis Kota Prabumulih. (Artikel) Pemerintah Kota Prabumullih. https://www.kotaprabumulih.go.id/geografis/
Pribadi, A., & Wiratmoko, M. E. (2019). Karakteristik madu lebah hutan (Apis dorsata Fabr.) dari berbagai bioregion di Riau. Jurnal Penelitian Hasil Hutan, 37(3), 185–200. https://doi.org/10.20886/jphh.2019.37.3.185-200
Rafi, M., Widyastuti, N., Suradikusumah, E., & Darusman, L. K. (2012). Aktivitas antioksidan, kadar kandungan fenol dan flavonoid total dari enam tumbuhan obat Indonesia. Jurnal Bahan Alam Indonesia, 8(3), 159–165.
Sasongko, D. D., & Junus, M. (2012). Hubungan antara ketinggian tempat sisiran sarang dengan panjang, lebar, tebal, dan bobot sisiran sarang lebah hutan (Apis dorsata) di hutan harapan PT. Reki Jambi. (Skripsi) Universitas Brawijaya Malang.
Shehu, A., Adzim, M., Rohin, K., Aziz, A. A., & Ismail, S. (2015). Antibacterial activity and antioxidant capacity of Malaysian tualang honey. International Journal of Science and Research, 4(4), 1758–1762.
Sjamsiah, Sikanna, R., Rifkah, A., & Saleh, A. (2018). Penentuan sifat fisikokimia madu hutan (Apis dorsata) Sulawesi Selatan. Al-Kimia, 6(2), 185–193. https://doi.org/10.24252/al-kimia.v6i2.6668
Sudarmadji, S., Haryono, B., & Suhardi. (2010). Analisa bahan makanan dan pertanian. Liberty Yogyakarta.
Suranto, A. (2007). Khasiat dan manfaat madu herbal. Jakarta: Agro Media Pustaka.
Triwanto, J., Herlina, K., & Muttaqin, T. (2021). Kualitas fisikokimia pada madu dari nektar bunga Randu (Ceiba pentandra) dan Kaliandra (Calliandra callothyrsus). Journal of Forest Science Avicennia, 4(1), 102–113.
Tyagita, N., Safitri, A. H., & Widyawati, E. (2021). Penuaan dan stres oksidatif. Fakutas Kedokteran Universitas Islam Sultan Agung Semarang.
Ustadi, Radiati, L. E., & Thohari, I. (2020). Komponen bioaktif pada madu karet (Hevea brasiliensis) madu kaliandra (Calliandra callothyrsus) dan madu randu (Ceiba pentandra). Jurnal Ilmu Dan Teknologi Hasil Ternak, 12(2), 97–102. https://doi.org//10.21776/ub.jitek.2017.012.02.6
Wilczyńska, A., & Ruszkowska, M. (2014). Water activity and colour parameters changes during storage of Linden and Buckwheat Honeys. Gdynia Marittime, 84(1), 84(1), 174–181.
Wulandari, D. D. (2017). Analisa kualitas madu (keasaman, kadar air, dan kadar gula pereduksi) berdasarkan perbedaan suhu penyimpanan. Jurnal Kimia Riset, 2(1), 2(1), 16–22. https://doi.org/10.20473/jkr.v2i1.3768
DOI: https://doi.org/10.24036/bsc.v7i2.125200
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