Analysis of Genetic Profiles of Heavy Metal Phytoremediator Plants From Gold Mining Areas

Sih Winarti, Liswara Neneng, Yohanes Edy Gunawan, Chaidir Adam

Abstract


The resistance and survival ability of phytoremediator plants in a polluted environment is thought to be a form of genetic adaptation. This research aimed: (1) to identify the dominant plant species in ex-gold mining area; (2) to analyze the genetic profile of phytoremediator plants from ex-gold mining area; and (3) to compare the genetic profile of heavy metal phytoremediator plants from ex-gold mining area with the same plant species from non-mining area. The samples of this study were Cyperus sp., Lycopodium sp., and Melastoma sp. The research procedures carried out include sample collection, DNA isolation, DNA amplification with PCR, and DNA visualization with Electrophoresis. The results show that the dominant plant species of ex-gold mining area are Cyperus sp., Lycopodium sp., and Melastoma sp. The genetic profile analysis of dominant plant species of ex-gold mining area show that no DNA bands appeared from the target gene as the result of amplification using specific primers of Metallothionein gene. The result of RAPD analysis using OPA-04 universal primers show that at 500-750 bp there are differences in DNA bands that appeared between the samples. DNA bands that appeared in the genetic profile of phytoremediator plants is thought to be the representation of the gene that responsible for heavy metals tolerance.

Keywords


Genetic Profile, Phytoremediator, Phytoremediation, Bioremediation, Heavy Metal, PCR

References


Anjum, Naser A. et al. 2015. “Jacks of Metal/Metalloid Chelation Trade in Plants—an Overview.” Frontiers in Plant Science 6(APR): 1–17.

Aziz, Amal A. et al. 2008. “Genetic Aspects of Heavy Metals Phytoremediation Abilities of Sunflower Plants.” Egypt. J. Genet. Cytol. 37(January 2008): 103–14.

Babaoğlu, Selcen, Leyla Açık, Nezaket Adıgüzel, and Şebnem Ellialtıoğlu. 2014. “RAPD-PCR Analysis of Hyperaccumulator Plants A . Corsicum and A . Murale Induced by Ni Treatment.” (June): 1–9.

Chiang, Huai-Chih, Jing-Chi Lo, and Kuo-Chen Yeh. 2006. “Genes Associated with Heavy Metal Tolerance and Accumulation in Zn/Cd Hyperaccumulator Arabidopsis Halleri: A Genomic Survey with CDNA Microarray.” Environmental Science & Technology 40(21): 6792–98. https://doi.org/10.1021/es061432y.

Chibuike, G. U., and S. C. Obiora. 2014. “Heavy Metal Polluted Soils: Effect on Plants and Bioremediation Methods.” Applied and Environmental Soil Science 2014: 1–12.

Clemens, Stephan, Eugene J. Kim, Dieter Neumann, and Julian I. Schroeder. 1999. “Tolerance to Toxic Metals by a Gene Family of Phytochelatin Synthases from Plants and Yeast.” EMBO Journal 18(12): 3325–33.

Dieffenbach, C. W., T. M.J. Lowe, and G. S. Dveksler. 1993. “General Concepts for PCR Primer Design.” Genome Research 3(3): 30–37.

Kachenko, Anthony G., Balwant Singh, and Naveen P. Bhatia. 2007. “Heavy Metal Tolerance in Common Fern Species.” Australian Journal of Botany 300(1): 207–19.

Kim, S et al. 2005. “Increased Nicotianamine Biosynthesis Confers Enhanced Tolerance of High Levels of Metals, in Particular Nickel, to Plants.” Plant Cell Physiology 46(11): 1809–18.

Koźmińska, Aleksandra, Alina Wiszniewska, Ewa Hanus-Fajerska, and Ewa Muszyńska. 2018. “Recent Strategies of Increasing Metal Tolerance and Phytoremediation Potential Using Genetic Transformation of Plants.” Plant Biotechnology Reports 12(1): 1–14. http://dx.doi.org/10.1007/s11816-017-0467-2.

Lopez-Bucio, J, MF Nieto-Jacobo, V Ramırez-Rodrıguez, and L HerreraEstrella. 2000. “Organic Acid Metabolism in Plants: From Adaptive Physiology to Transgenic Varieties for Cultivation in Extreme Soils.” Plant Sci 160(1): 1–13.

Neneng, Liswara. 2009. Aplikasi Konsorsium Mikroorganisme dan Tumbuhan Fitoremediator Merkuri (Hg) untuk Reklamasi Lahan Pasca Penambangan Emas di Kalimantan Tengah. Laporan Akhir Penelitian Hibah Stranas DIKTI.

Sanità Di Toppi, L., M. N. V. Prasad, and S. Ottonello. 2002. “Metal Chelating Peptides and Proteins in Plants.” In Physiology and Biochemistry of Metal Toxicity and Tolerance in Plants, Springer, Dordrecht, 59–93.

Syamsurizal, S., Handayani, D., Kadri, H., & Badriyya, E. (2019). Genotyping SNP rs7903146 TCF7L2 gene for detection T2DM in Indonesian melayu ethnic. Journal of Physics: Conference Series, 1317(1), 12090. IOP Publishing.

Torgersen, Christian E., Colden V. Baxter, Hiram W. Li, and Bruce A. McIntosh. 2006. “Landscape Influences on Longitudinal Patterns of River Fishes: Spatially Continuous Analysis of Fish-Habitat Relationships.” American Fisheries Society Symposium 2006(48): 473–92.

Tripathi, P et al. 2015. “Recent Advances in the Expression and Regulation of Plant Metallothioneins for Metal Homeostasis and Tolerance In: Environmental Waste Management.” CRC Press, Boca Raton: 551–64.

Winarti, Sih., and Neneng, Liswara. 2018. Analisis Profil Genetik Tumbuhan Fitoremediator Logam Berat dari Areal Bekas Tambang Emas di Kabupaten Gunung Mas. Laporan Akhir Penelitian PNBP UPR 2018




DOI: https://doi.org/10.24036/0202041108305-0-00

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