Antibacterial Activities of Kombucha Tea From Some Types of Variations of Tea on Escherichia coli and Staphylococcus aureus
Abstract
Tea can be used as a fermented drink called kombucha tea. Based on the oxidation process tea is classified into white tea, green tea, oolong tea and black tea. The compounds produced from the fermentation of kombucha tea have a fairly good antibacterial effect. This study aims to determine the antibacterial activity of kombucha tea fermentation from several variations of tea types against Escherichia coli and Staphylococcus aureus. This research is an experimental study with a completely randomized design (CRD) with 5 treatments and 3 replications conducted in May-June 2019 at the Microbiology Laboratory and the Integrated Research Laboratory of the Department of Biology FMIPA UNP. Kombucha tea used in this study was white tea, green tea, oolong tea and black tea. Antibacterial activity test was carried out by paper disc diffusion method against microbial test of S. aureus and E. coli bacteria. Research results showed that the highest antibacterial activity of kombucha tea fermentation in E.coli was found in black tea with a value of 1.64 cm and the lowest in tea white with a value of 1.04 cm. In S. aureus the highest value was obtained in black tea with a value of 2.48 cm and the lowest in white tea with a value of 1.39 cm.
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Ahmed SE. 2003. Biochemical and microbial changes during fermentation of tea fungus (kombucha).Thesis. Sudan: University of Khartoum.
Ardheniati, M. 2008. Kinetika Fermentasi Pada Teh Kombucha dengan Variasi Jenis Teh Berdasarkan Pengolahannya. Skripsi. FP. Surakarta: Universitas Sebelas Maret
Battikh, H., Chaieb, K., Bakhrouf, A., & Ammar, E. 2011. Antibacterial And Antifungsional Activities of Black and Grean Kombucha Teas. Journal of Food Biochemistry. 37 : 231-236.
Bronzwaer, SL., Cars, O., Buchhols, U., Molstad, S., Goettsch, W. 2002.A European Study on The Relationship between Antimicrobial Use and Antimicrobial Resistance. Emerging Infectious Disease, 8 : 278-282.
Brooks, G.F., Janet, S.B., Stephen A.M., Jawetz, Melnick & Adelbergs. 2001. Mikrobiologi Kedokteran. Alih Bahasa oleh Mudihardi, E., Kuntaman, Wasito, E.B., Mertaniasih, N.M., Harsono, S., dan Alimsardjono, L. Jakarta : Penerbit Salemba Medika.
Davis, W.W. & T.R Stout. 1971. Disc plate methods of microbiological antibiotic assay. J. Microbiology. (4):659-665.
Greenwalt, C. J., R. A. Ledford & K. H. Steinkraus. 1998. Detoxification and Characterization of The Antimikrobial Activity of The Fermented Tea Kombucha. John Wiley and Sons. Inc. New York.
Haetamin, K., Abun, dan Y. Mulyani. 2008. Study Pembuatan Probiotik (Bacillus liecheniformis, Aspergillus niger, dan Saccharomyces cereviseae) sebagai feed Supplement serta Implikasinya terhadap Pertumbuhan Ikan Nila Merah. Laporan Penelitian. Fakultas Perikanan dan Ilmu Kelautan. Universitas Padjadjaran.
Jambang, N. 2004. Studi Aktivitas Antibakteri dan Antioksidan Pada Beberapa Merk Teh Hitam yang Beredar Di Pasaran Kota Malang. Skripsi. Malang : Fakultas Teknologi Pertanian Universitas Brawijaya.
Jayabalan, R., Marimuthu, S. & Swaminathan, K. 2007. Changes in content of organic acids and tea polyphenols during kombucha tea fermentation. Food Chemistry, 102: 392-398.
Martina, L., Ebenezar, A., & Ghani, M. 2013. An in vitro comparative antibacterial study of different concentrations of green tea extracts and 2% chlorhexidine on Enterococcus faecalis. Saudi Endodontic Journal. 3(3): 120-23.
Rinihapsari, E. & Catur A. R. 2008. Fermentasi Kombucha dan Potensinya sebagai Minuman Kesehatan. Media Farmasi Indonesia. Vol. 3. No. 2. Hal: 241-246.
Rofiq, M.N. 2003. Potensi suspensi teh fermentasi kombucha (STK)pengaruh kombucha dalam mengontrol infeksi Salmonella sp dan pengaruhnya terhadap performans ayam broiler. Tesis.Bogor: Institut Pertanian Bogor.
Sousa de, C. P. 2006. Escherichia coli as a Specialized Bacterial Pathogen. Revista Biologis E. Ciencias Terra, 6 (2): 341-352.
Simanjuntak, R.M.J. dan H, Mutiara. 2016. Pengaruh Pemberian Teh KombuchaTerhadap Pertumbuhan Salmonella Typhi. Majority, 5 (5): 48-54.
Sreeramulu, G.Y. dan Knol, W. 2000. Kombucha Fermentation and It’s Antimikrobal Activity. Journal Agriculture Food Chemistry. 886, 65-73.
Volk WA. 1992. Basic Microbiology. New York: Harper Collins Publisher.
Zeniusa, P. dan M. R. Ramadhian. 2017. Efektifitas Ekstrak Etanol Teh Hijau dalam Menghambat Pertumbuhan Escherichia coli. Majority, 7(1): 26-30.
DOI: https://doi.org/10.24036/0202042105679-0-00
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