PENGARUH PENGENCER PBS TERHADAP KUALITAS SPERMA AYAM KUKUAK BALENGGEK UNTUK INSEMINASI BUATAN

Abstract

The balenggek chickens has been raised by rural communities for many generations in Payung Sakaki, Tigo Lurah, and Pinang Awan, Regency of Solok, Province of West Su matera.The Balenggek chicken had low sperm concentration per ejaculated. This study was carried out to identify the influence of PBS as suspender to the sperm quality of ba lenggek chickens for Artificial Insemination (AI). Sperm collected by massage technique and suspended in physiologic solution (NaCl 0,9%) or PBS (Phosphate Buffer Saline) with sperm concentration 200 million sperm/ ml suspender. Suspended sperm were places in Cool Case (-4°C), and Room Temperature (26-28°C) for 2 or 4 hours before AI. The egg that resulted in AI was incubated to analysis the egg fertility and hatchability. The results shows there were no significantly higher (p<0,05) to all treatments. The eggs fertility 85-90% and the eggs hatchability was 65-70% in both treatments for all temperature condi tions. It can conclude that PBS can suspender and preserve the Balenggek chicken sperm quality for AI.

Keywords: balenggek chickens, PBS, sperm quality, artificial insemination