Optimization of Medium Fermentation for Production of Antimicrobial Compounds by Endofit Bacteria Andalas Plant (Morus macroura Miq.) B.J.T.A-6 Isolate

Nada Nafion(1), Dwi Hilda Putri(2), Irdawati Irdawati(3),
(1) Jurusan Biologi, FMIPA, Universitas Negeri Padang  Indonesia
(2) Jurusan Biologi, FMIPA, Universitas Negeri Padang  Indonesia
(3) Jurusan Biologi, FMIPA, Universitas Negeri Padang  Indonesia

Corresponding Author
Copyright (c) 2019 Nada Nafion

DOI : https://doi.org/10.24036/0201931102865-0-00

Full Text:    Language : en    Language : en    Language : en
Submitted : 2019-01-23
Published : 2019-04-01

Abstract


Cases of bacterial resistance to antibiotics are discussed with serious problems in the world of health. New antimicrobial compounds are needed which are more effective in treating infectious diseases. Isolate B.J.T.A-6 is an endophytic bacteria from Andalas plants (Morus macroura Miq.) which is known to be able to produce antimicrobial active compounds. Antimicrobial compounds can be produced by growing them on fermented media. The purpose of this study was to optimize the medium fermentation of Andalas endophytic bacteria of B.J.T.A-6  isolates in producing antimicrobial compounds. While medium fermentation is Nutrient Broth (NB), Muller Hinton (MH), and Luria Bertani Broth (LB). Antimicrobial activity tests were carried out by means of diffusion. The parameters used were the diameter of the inhibition zone against Staphylococcus aureus around the disc paper. The profile of medium fermentation optimization was analyzed statistically. The results showed that B.J.T.A-6  isolates could inhibit the highest growth of S. aureus by using LB fermentation medium. 


Keywords


Endofit, Bacteria, fermentation, optimization, Medium

References


Afifah N, Putri DH, & Irdawati. 2018. Isolation and Identification of Endophytic Bacteria from the Andalas Plant Stem (Morus macroura Miq.). Bioscience, 2(1): 72-75.

Das M P, Bhowmick M, & Reynolds M. 2016. Optimization of Culture Conditions for Production of Antibacterial Metabolite by Marine Bacteria. Journal Pharmaceutical Sciences Review and Research, 38(2), 5.

Mangamuri UK, Vijayalakshmi M, Poda S, & Agasar D. 2014. Optimization of the cultural parameters for improved production of antimicrobial metabolites by Streptomyces gulbargensis DAS 131. British Journal of Pharmaceutical Research, 4(9), 1130.

Pemerintah Indonesia. 2011. Peraturan Mentri Kesehatan Republik Indonesia Nomor 2406 Tentang pedoman Umum Penggunaan Antibiotik. Mentri Kesehatan. Jakarta.

Prescott H. 2002. Laboratory Exercises in Microbiology Fifth Edition. USA: The Mc Graw Hill.

Shukla S, Shukla H, Pandey AK. 2013. Optimization Of Various Parameters For Production Of Antimicrobial Compounds By Fusarium Roseum Fgcc61. Journal of Pharmacy and Pharmacheutical Sciences, 3(12), 16.

Sulistyani N, & Narwanti I. 2015. Aktivitas Cairan Kultur Bakteri Penghasil Antibiotik (Isolat P301) terhadap Staphylococcus aureus ATCC 25923 dan Optimasi Waktu Produksi Metabolit Sekunder. Jurnal Kefarmasian Indonesia, 13(2): 181-186.

Syamsuardi, Jamsari, & Jawati S. (2005). Diversitas Morpologis & Genetik Pohon Andalas (Morus Macroura Miq.), Flora Identitas Sumatera Barat, dan Pemanfaatannya Secara Berkelanjutan. Paper presented at the Improving Appre Prosiding Workshoop Ciation and Awareness On Concervation Of High Value Indigenous Wood Species of Sumatera, Pekanbaru.

Talluri P, & Lanka SS. 2017. Optimization of Cultural Parameters for the Production of Antimicrobial Compound from Lactobacillus Fermentum (MTCC No. 1745). Journal of Bacteriology & Mycology, 4(5), 4.

Todar K. 2007. Nutrion and growth of bacteria. University Wisconsin: Madison Department of Bacteriology.


Article Metrics

 Abstract Views : 71 times
 Untitled Downloaded : 0 times  PDF (Bahasa Indonesia) Downloaded : 24 times  PDF Downloaded : 11 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Nada Nafion

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.